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Single Molecule Biology download pdf ebook

نویسنده: Jovanni Stevens   

Single Molecule Biology




Author: Alexander E. Knight
Type: eBook
Date Released: 2008
Format: pdf
Language: English
Page Count: 353
Isbn10 Code: 0123742277
Isbn13 Code: 9780123742278


Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective. The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. Among the topics covered are enzymes, motor proteins, membrane channels, DNA, ribozymes, cytoskeletal proteins, and other key molecules of current interest. An introduction by the editor provides a concise review of key principles and an historical overview. The last section discusses applications in molecular diagnostics and drug discovery. * Organized by biological system or molecule. * Each chapter discusses insights into mechanism of action, structure, and function * Covers enzymes, motor proteins, membrane channels, DNA, ribozymes, etc. * Includes an introduction to key principles and an historical overview. * Discusses applications in molecular diagnostics and drug discovery. * Provides an expert's perspective on future developments. From the Back Cover Single molecule techniques are powerful tools for the dissection of biological function. They can be used to investigate the mechanisms of molecular machines, wind up single strands of DNA, or unravel individual proteins. They can detect the flickering open of an ion channel in a membrane or watch an enzyme turn over a molecule of substrate. This book reveals how single molecule techniques have revolutionized our understanding of biology at the nanoscale-and it is the first book to be focused on the biology, rather than the techniques themselves. As such, it is targeted at biologists and a broad interdisciplinary readership. Each chapter in this multicontributed book is written by an expert in the field, covering a key biological system studied by single molecule methods, and exploring what has been learnt from this new approach. Systems examined include molecular motors (kinesin, dynein, myosin, and the bacterial flagellar motor); nucleic acids (chromatin, DNA-based enzymes, and the ribosome); membranes and ion channels; and a look at applications to microarray technology.

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